by C. Rose Kennedy
figures by Kaitlyn Choi
What’s in a Flavor?
The word “flavor” pervades our daily vocabulary, evoking associations of rich or vivid experiences beyond the experience of eating. Even in the literal context, the Flavor Extract Manufacturers Association (FEMA) describes flavor as “the entire range of sensations that we perceive when we eat a food or drink a beverage. Flavor encompasses a substance’s taste, smell, and any physical traits we perceive” . While foods do intrinsically contain flavoring substances, 90% of Americans’ grocery purchases also contain added flavors . These flavors are used to amplify or modulate the sensory experience associated with existing qualities of a product. Furthermore, they may also be used to make healthy yet bland options (like those lacking an excess of sugar or trans-fats) more appealing. For example, flavor agents may make reduced-fat foods seem rich and creamy, or add salty zest to low-sodium products. It’s obvious that flavors have immense power to influence our gustatory experiences, but what are they and where do they come from?
All flavors, including those intrinsically present in food, as well as natural and artificial flavor additives, are small chemical compounds or mixtures of compounds (Figure 1). While chemicals often evoke negative associations, it’s important to remember that all of the components of our food, our bodies, and the world around us are actually chemicals. Even things like water (dihydrogen monoxide), table sugar (sucrose, or alpha-D-glucopyranosyl-(1à2)-beta-D-fructofuranoside), and baking soda (sodium bicarbonate) are chemical compounds. In fact, the chemical properties and structures of flavoring substances are exactly what enable them to react with each other during cooking (think of that distinctive grilled flavor) bind with the taste receptors in our mouths.
The FDA broadly defines natural flavors to include any flavor isolated from natural sources like plant material (fruits, roots, bark, herbs, etc.) or animal products (meat, dairy, etc.) . Artificial flavors are any flavors that are not defined as natural, even if they have the exact same chemical composition as flavors isolated directly from nature . This distinction between the origins of flavors has no bearing on how safe, healthy, or delicious they are. In fact, flavor agents produced in a controlled laboratory setting undergo rigorous quality control at every stage, and do not require a lengthy, labor- and resource-intensive extraction process or acquisition of naturally rare or difficult-to-cultivate materials. As such, many nature-identical artificial flavors are actually available in higher purity than their natural congeners and may be obtained with less damage to the environment .
These artificial flavors are prepared by an elite group of highly trained professionals known as flavorists or flavor chemists, who must proceed through a rigorous seven-year apprenticeship (following university education) before they can become certified by the Society of Flavor Chemists [5, 6]. It is estimated that there are only roughly 500 certified flavorists worldwide . Flavorists prepare mixtures of flavors to meet consumer flavor preferences, all while following a stringent set of safety and efficiency guidelines. Furthermore, because they are considered ingredient manufacturers flavorists and flavor production companies must comply with all the same FDA and USDA rules developed for food production .
To aid this process, a panel of scientific and medical experts (representing fields including chemistry, toxicology, pharmacology, medicine, pathology, and statistics) serving the Flavor Extract Manufacturers Association (FEMA) has compiled a list of thoroughly vetted flavor compounds that are “generally recognized as safe (GRAS)” . The FEMA Expert Panel communicates their findings and all scientific data with the FDA, with international scientific and regulatory bodies, and in the scientific literature [8, 9]. Flavor companies are not required to disclose application of flavor ingredients that have already passed this rigorous review, but any novel flavor substances that have not yet made it onto the list require rigorous testing and approval to demonstrate safety before they may be incorporated into consumer products.
Let’s consider a few cases of flavor chemistry in action!
Vanilla is the most popular flavor worldwide. Not only is it used in favorites like ice cream, candies, cakes, and cookies, but vanilla also enhances perception of sweetness and other flavors (like those in chocolate, coffee, fruit, and nuts) .
Natural vanilla extract is obtained by steeping vanilla beans in alcohol to afford a solution of vanillin and other minor components that may be used in cooking and baking. While as many as 250 taste and aroma components have been detected in vanilla extract, most of these are destroyed upon exposure to elevated temperature, causing the complexity of the vanilla flavor to diminish upon baking. Furthermore, vanilla beans can only be obtained from hand-pollinated flowering orchids in a handful of tropical areas, making them precious commodities, especially given the vast demand for vanilla (over 16,000 metric tons annually) worldwide . To meet this demand, flavor companies like International Flavors and Fragrances, Inc. and Givaudan have entire production lines devoted to development of high-quality substitutes for natural vanilla extract , .
One of these substitutes is castoreum extract. Castoreum is a secretion from the anal glands and castor sacs of beavers, which use it to mark their territorial claims. Castoreum extract possesses a warm, sweet odor and may be used as a stand-in for vanilla extract in many dairy products and baked goods . Following rigorous evaluation, castoreum and its extracts have earned a place on the GRAS List; however, worldwide consumption is relatively low (only about 300 pounds annually). It is interesting to note, though, that because castoreum is extracted from an animal source, it is considered a natural flavor, not an artificial flavor.
The bulk of vanilla substitutes are actually nature-identical vanillin and vanillin derivatives synthesized on multi-ton scale from guaiacol or lignin extracts, which can be isolated from wood pulp (Figure 2) , . In addition to providing an inexpensive source of vanillin—in higher purity than one could obtain from natural vanilla extract—this synthesis enables production of vanillin derivatives possessing desirable qualities, like increased flavor potency and thermal stability. For example adding one extra carbon atom to vanillin affords ethyl vanillin, which is 2–4 times more flavorful than vanillin itself. Due to this desirable property and its long-standing status on the GRAS list, ethyl vanillin has been used in some vanilla flavoring since the 1930s.  Perhaps it should come as no surprise, then, that in blind taste-tests, tasters preferred the artificial vanilla flavoring (including industrially produced vanillin and its analogues) in treats like cookies that must be baked at the high temperatures that cause many of the minor components of natural vanilla extract to decompose .
Butter Me Up
The rich, smooth flavor of butter is another consumer favorite, adding body and depth to cooking sprays, sauces, baked goods, and (of course) popcorn. This flavor arises from the production of diacetyl and acetoin as byproducts of sugar fermentation in the production of cultured dairy products like cultured butter, buttermilk, and sour cream .
While small quantities of these compounds can be isolated from these consumer-based dairy products, the very low efficiency of the extraction limits its practicality. Instead, these flavor additives are generally produced through chemical synthesis or industrial fermentation on specialized culture, which is optimized for flavor production, but is not the sort of media people generally like to eat (Figure 3) . When produced through this culturing technique, diacetyl and acetoin are listed as “natural butter flavoring,” but when they are produced through controlled laboratory synthesis, the very same components are listed as “artificial butter flavoring” .
You may have heard reports about “popcorn worker’s lung,” in which a handful of cases of lung disease have been linked to inhalation of very high levels of diacetyl in some microwave popcorn production facilities . However, both diacetyl and acetoin are long-standing members of the GRAS list. It is essential to remember that a high dose of anything can be toxic! For example, there have been numerous cases of deaths related to overdosing on water (not drowning, actually just drinking far too much) , . The FDA and OSHA have investigated concerns regarding aerosolized diacetyl carefully and have developed new standards to limit exposure to airborne particles in occupational settings . Additionally, their reports reiterate that the levels to which general consumers are exposed is far below the risk threshold. Furthermore, ingesting flavor agents like diacetyl routes these substances to the stomach (rather than the lungs), which is perfectly equipped to process these molecules. So go ahead, have your butter (flavor) and eat it too!
A Flavor Like You’ve Never Dreamed
As a few of our classic flavor favorites illustrate, flavor chemistry is both an art and a science dedicated to safely producing robust and resource-efficient natural and artificial flavors for your enjoyment. Perhaps the most exciting aspect, however, is the opportunity to combine known flavors in novel ways to explore flavors beyond what we’ve ever experienced. Flavorists seek to identify and fill “white spaces,” which are combinations of flavor qualities that consumer may enjoy but are unrepresented in products on the market. So next time you try an out-of-this-world treat like Juicy Fruit™ gum, a novel jelly bean, or your favorite sports drink, remember the labor of love that went into its development . For in the memorable words of Willy Wonka: “Invention, my dear friends, is 93% perspiration, 6% electricity, 4% evaporation, and 2% butterscotch ripple.”
C. Rose Kennedy is a Ph.D. candidate in the Department of Chemistry and Chemical Biology at Harvard University.
 Flavor Facts. Flavor Extract Manufacturers Association (FEMA) http://www.flavorfacts.org (accessed August 23, 2015)
 “The Tastemakers” La Gorce, T. New Jersey Monthly. January 17, 2011. http://njmonthly.com/articles/eat-drink/the-tastemakers/ (accessed August 23, 2015)
 “Section 101.22 Foods; labeling of spices, flavorings, colorings and chemical preservatives.” Code of Federal Regulations. Title 21 – Food and Drugs. Food And Drug Administration, Department Of Health And Human Services. April 1, 2010. http://www.gpo.gov/fdsys/pkg/CFR-2010-title21-vol2/xml/CFR-2010-title21-vol2-sec101-22.xml (accessed August 23, 2015)
 “What is the difference between artificial and natural flavors?” Reineccius, G. Scientific American. July 29, 2002. http://www.scientificamerican.com/article/what-is-the-difference-be-2002-07-29/ (accessed August 23, 2015)
 The Society of Flavor Chemists. http://flavorchemists.com/ (accessed August 23, 2015)
 “You’re a what? Flavorist” Green, K. Occupational Outlook Quarterly. Winter 2004–2005. US Bureau of Labor Statistics http://www.bls.gov/careeroutlook/2004/winter/yawhat.pdf
 “The Taste Makers” Khatchadourian, R. The New Yorker. November 23, 2009. http://www.newyorker.com/magazine/2009/11/23/the-taste-makers (accessed August 23, 2015)
 “Flavor Safety: Myth vs. Fact” Mat. Flavor Facts. Flavor Extract Manufacturers Association (FEMA) June 25, 2015. http://www.flavorfacts.org/flavor-safety-myth-vs-fact/ (accessed August 23, 2015)
 “How U.S. FDA’s GRAS Notification Program Works” Gaynor, P. Food Safety Magazine, December 2005/January 2006. http://www.fda.gov/Food/IngredientsPackagingLabeling/GRAS/ucm083022.htm#authors (accessed August 23, 2015)
 “Vanilla Extract” Cook’s Illustrated. March 1, 2009. http://www.cooksillustrated.com/taste_tests/455-vanilla-extract (accessed August 23, 2015)
 Always Vanilla. International Flavors and Fragrances, Inc. http://alwaysvanilla.com/ (accessed August 23, 2015)
 Anything but Plain Vanilla. Givaudan TasteEssentials Vanilla. https://www.givaudan.com/flavours/world-flavours/tasteessentials/vanilla (accessed August 23, 2015)
 “Castoreum” Burdock, G. A. Fenaroli’s Handbook of Flavor Ingredients. CRC Press, 2005. 276–278
 “Vanillin: Synthetic Flavoring from Spent Sulfite Liquor.” Hocking, M. B. Journal of Chemical Education, 1997, 74, 1055. DOI: 10.1021/ed074p1055
 “Vanillin” Vidal, J.-P. Kirk-Othmer Encyclopedia of Chemical Technology, 2006, DOI: 10.1002/0471238961.2201140905191615.a01.pub2
 “Ethyl Vanillin” Burdock, G. A. Fenaroli’s Handbook of Flavor Ingredients. CRC Press, 2005. 639–640.
 Pavia et al. Introduction to Organic Laboratory Techniques (4th ed.). ISBN 978-0-495-28069-9.
 “Production of Diacetyl and Acetoin by Lactic Acid Bacteria” Oberman, H., Piątkiewicz, A.; Libudzisz, Z. Nahrung, 1982, 26, 615. DOI: 10.1002/food.19820260706
 “Biotechnological Production of Flavours and Fragrances” Krings, C.; Berger, R. G. Applied Microbiol Biotechnology. 1998, 49, 8.a
 “Beware Diacetyl: The Next Legal Quagmire” Fama, R. Food Safety Magazine. October/November 2007. http://www.foodsafetymagazine.com/magazine-archive1/octobernovember-2007/beware-diacetyl-the-next-legal-quagmire/ (accessed August 23, 2015)
 “Hyponatremia” Adrogué, H. J.; Madias, N. E. New England Journal of Medicine 2000, 342, 1581. DOI: 10.1056/NEJM200005253422107
 “Death from hyponatremia as a result of acute water intoxication in an Army basic trainee.” Garigan, T. P.; Ristedt, D. E. Military Medicine,1999, 164, 234.
 “NIOSH Alert: Preventing Lung Disease in Workers Who Use or Make Flavorings,” DHHS (NIOSH) Publication No. 2004-110. http://www.cdc.gov/niosh/docs/2004-110/ (accessed August 23, 2015)
 “Sweet Memories: How Jelly Belly Invents Flavors” Greenwood, V. The Atlantic. August 17, 2010. http://www.theatlantic.com/health/archive/2010/08/sweet-memories-how-jelly-belly-invents-flavors/61477/ (accessed August 23, 2015)
31 thoughts on “The Flavor Rundown: Natural vs. Artificial Flavors”
The caption to figure 1 is wrong. Flavor, or aroma, molecules do not bind to receptors in the mouth. They bind to smell receptors in the nose. Taste molecules, like sugar, bind to receptors in the mouth.
I guess in future, we will have artificial fiber powders and sticks that can taste anything.
Thank you for this article. Was able to share this with my chemistry class.
Wow, this is devastatingly biased. The article was clearly bought and paid for by the Flavor Industry. Shamefully so. I expect better of something coming out of Harvard. Much better. It is so “Pro-Chem” and “Let’s leave nature alone….”. You know: Better Living Through Chemicals, because, after all, even “natural humans” are just bags of chemicals. So, chemicals MUST be good for you….. The industry is thinking ONLY of your health and welfare. Yeah, that’s it. Eat up!
Shocked and disappointed.
I can assure you that no one paid us to write this article. We’re a bunch of grad students, not professional writers, who earn absolutely no profit off of our articles.
This is FAKE NEWS.
good point thats what i would say
I don’t see the point of this rant. If a so-called natural product is chemically identical to an artificial product, it doesn’t matter which one you ingest. Moreover, there are plenty of harmful natural products and plenty of harmless artificial products.
Oh please. Your unstable anxiety about chemicals in the modern world won’t do you any good. Oh, I know you avoid BPA, buy organic, and won’t buy anything that says “all-natural” but this doesn’t make you an expert, it makes you a pernicious, lie-spouting alarmist. You avoid BPA so test you levels of BPB and BPC. They’ll be just as high. You pay more for worse food because you avoid pesticides and nitrate fertilizer, but end up with more contaminants due to a lack of sterilization. Now you’re picking on artificial flavors. The best thing humans have ever done. I love my distilled fruity essences, especially the homogenized single compound varieties.
Oh hello M’Lady
I just learned about esterification in my organic chem class and I think it’s fascinating. Considering the trajectory we are on regarding the environment, we might not even be able to GET natural flavors, etc in 100 years so at least we have a back up.
Also to everyone freaking out about the artificial chemical aspect, unless you are super rich folks who can buy 100% unprocessed foods, build all your own furniture, don’t use internet, etc (basically amish) you have chemicals to thank for about 90% of your life already…. Like the advancement in technology that led to the computer, laptop, tablet, or phone you’re using to write these comments. 😉
I found this super helpful, for my high school chemistry class that I’m writing a paper for, thanks. I love the images/diagrams.
I’m still getting over the fact that at some stage in my life I could have eaten the secretion of a Beavers anal gland…I guess someone got too close to a Beaver one day, and then thought wouldn’t that be nice in a vanilla slice…
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Just because the FDA/USDA/FEMA has designated natural and artificial flavors as “generally recognized as safe” it is misleading to say that they are truly safe. It just means we do not yet have the data to prove they are harmful. There are so many things we used to think of as safe that we now know are carcinogenic. An example that comes to mind is the pesticide DDT, which in the 1940s was sprayed into public pools while children were swimming in them, and we now know is a carcinogen. Until there are multiple large longitudinal randomized control trials (which is likely impossible to perform, let alone find someone to fund that research) that prove there is truly no significant difference in health outcomes associated with consumption of natural and artificial flavors you cannot conclude that natural and artificial flavors are “safe”. We simply do not have the data to make that claim.
‘Just because FDA’…I could’ve stopped reading there but I was intrigued. What impresses me most is that FDA approved foods go through rigorous testing, no matter how and where they are produced. Do you know what is used to preserve food? It is impossible to buy food and store without chemicals because the only other way to eat “natural food” is to live in a jungle.
Sorry for the necropost but I felt someone will have this question and that, that someone will look for answers in and around here.
Hello, thank you for sharing this article it will be really helpful for me to understand about the flavor rundown natural vs artificial flavors. I appreciate your step for choosing this topic. Great article!! You have done a good research I must say, thank you very much for sharing this article.
Welp, this dosent answer my question of what artificial flavoring is in my ice cream. but i liked it! Helloooooo!!!! 🙂
So, I was looking up what the “natural flavor” is in the new “bubly” drinks, when I came across this article. I typically drink water and milk, and have an occasional Reed’s Gingerbrew ginger ale , but was curious when I saw this at the store today. Thanks for the info.
About 20 years ago, I went through a month of terrible itching 24/7. When I finally got an allergy test done, it showed that I’m allergic to artificial dyes (all but the two red dyes were the worst) and fragrances as well as some botanicals. After I stopped taking the “red” multi vitamin, eating my favorite red jolly ranchers, and started using fragrance free in everything, within two days, the itching stopped. Aren’t most artificial dyes made from petroleum? They were 20 years ago when I did my research at that time. Plus, my some of adhd focus and impulsivity improved after that as well. I’m thankful that I have been able to buy foods that don’t have artificial (OR natural flavors that I can’t find out what they really are) so I can live without dealing with the hives. It’s worth spending the extra time and $ to eat foods that don’t have these chemicals in them.
Thanks for taking the time to discuss this, I feel strongly about it and love learning more on this topic. If possible, as you gain expertise, would you mind updating your blog with extra information? It is extremely helpful for me.
This article reads as heavily corporate interest biased, even from Harvard.
Great article. Thank you for sharing the article about the flavor rundown natural vs artificial flavors.
I read the blog and I love the ice cream recipe. Thank you for sharing.
Good and informative article illustrated with beautiful images. Loved this one.
I’m getting hungry now. I’d love a big bowl of beaver anal gland secretion ice cream!